Core 2: Nuclear Magnetic Resonance
Core 2 Services
Core 2 provides a range of services for SECIM users.
For further details on prices and to request services, CLICK HERE. NMR Services include:
- Global metabolomics using standard 1D and 2D 1H detection for solutions (serum, urine, extracts, cultures, etc.). These utilize conventional 5-mm NMR tubes with sample volumes approximately 600 μL and a 600 MHz 5-mm cryogenic probe.
- Tissue metabolomics using high resolution magic angle spinning (HR-MAS). This utilizes a 4-mm Bruker HR-MAS probe operating at 600 MHz. HR-MAS provides high resolution data without extraction, thus providing a link between in vitro and in vivo measurements.
In addition, Core 2 is actively developing the following areas:
- Biomarker identification using both automated and manual approaches using 2D NMR and MS/MS with Core 3. In addition to conventional 5-mm NMR probes, we have a 1-mm HTS NMR probe that has the highest possible mass sensitivity for extremely mass limited samples.
- Automatic mixture analysis using COLMAR. We are working with Prof. Brüschweiler on the development of new capabilities and improved database matching of mixtures.
- Improved joint analysis of NMR and LC-MS datasets for more robust biomarker identification. This is in collaboration with the Nicholson/Holmes group at Imperial College and with SECIM Core 1 and Core 4.
- 13C detection of both natural abundance 13C and isotopically enriched samples. 13C detection offers many advantages over conventional 1H detection, primarily through the large chemical shift dispersion of 13C. These capabilities utilize a novel 1.5-mm HTS NMR probe that is optimized for 13C detection. Sample volumes are between 35 and 40 μL.
- Synthesis and characterization of DNP substrates. In collaboration with the Prestegard lab, we are working to expand the range of substrates for Dynamic Nuclear Polarization (DNP) NMR studies. Dissolution DNP can provide over 4 orders of magnitude enhancement of an NMR signal for a transient measurement in vivo. The goal of this SECIM offering will be first identify relevant metabolic pathways using some of the standard approaches in solution using SECIM cores. Then, we will attempt to synthesize and test for DNP a substrate that will allow users to probe mechanisms in vivo. The DNP measurements will be made by our partner, the NHMFL AMRIS facility.
- Isotopic flux measurements. For SECIM user studies that indicate specific defects in the TCA cycle, we will refer interested users to the Advanced Imaging Research Center at the UT Southwestern Medical Center for in vivo flux analysis using 13C isotopomers.
Core 2 Partners
Arthur Edison, University of Georgia
Rafael Brüschweiler, Ohio State University
Jim Prestegard, University of Georgia