Core 1: Mass Spectrometry Services

Core PI: Tim Garrett (tgarrett@ufl.edu)

Co-PI: Stephen Gardell (sgardell@SBPdiscovery.org)

Core 1 Services

Core 1 provides high-throughput global and targeted metabolomic and lipidomic analysis. For further details on prices and to request services, CLICK HERE.

Global metabolomics and/or lipidomics is performed using state-of-the-art high resolution mass spectrometry (Thermo Q-Exactive) coupled with ultra-high performance liquid chromatography (UHPLC) on serum, plasma, urine, tissue, cells, stool and other samples.

Targeted metabolomics offerings include:

  • Acylcarnitines
  • Amino acids
  • Organic acids
  • Malonyl CoA and Acetyl CoA
  • Pyridine and Adenine Nucleotides: Oxidized (13) OR Reduced Panels (NADH and NADPH)
  • Pyridine and Adenine Nucleotides: Oxidized AND Reduced Panels (15)
  • Tryptophan Metabolites
  • 1-Carbon metabolites

These are performed for a variety of matrices including cellular extracts, plasma or tissue using mass spectrometry.  New assays will be developed based on:

  • Demand and/or interest in other metabolite subsets and
  • Follow-up method development to validate putative metabolites identified by prior global metabolomics pursuits.

The targeted metabolomics employs MS with selected reaction monitoring (SRM) using either a direct infusion approach or after chromatographic separation (UHPLC or GC).

Biomarker identification is performed with accurate mass and fine mass resolution, tandem MS (MS/MS) provided by Core 3 and existing metabolomic databases (Metlin, ChemSpider, pubChem). Through constant collaboration, SECIM continues to grow the library of known metabolites.

Special emphasis in Core 1 is placed on developing SOPs for proper collection/storage of specimens and sample processing prior to MS and NMR. However, interaction with Core 3 provides a mechanism to improve the overall global metabolomics platform as new technologies are developed. An example of this is Isotopic Ratio Outlier Analysis (IROA), a collaborating technology offered through SECIM services.

Schedule a consultation or email secim-admin-l@lists.ufl.edu for more information.

Core 1 Partners

Chris Beecher, IROA Tech

Core 1 Facilities

Timothy J. Garrett Laboratory

Sanford Burnham Prebys Medical Discovery Institute – Targeted Metabolomics

CTSI Biorepository