SECIM provides high-throughput global and targeted metabolomic and lipidomic analysis. For further details on prices and to request services, CLICK HERE.
Core PI: Tim Garrett (tgarrett@ufl.edu)
Co-PI: Stephen Gardell (stephen.gardell@flhosp.org)
Global metabolomics and/or lipidomics is performed using state-of-the-art high resolution mass spectrometry (Thermo Q-Exactive) coupled with ultra-high performance liquid chromatography (UHPLC) on serum, plasma, urine, tissue, cells, stool and other samples.
Targeted metabolomics offerings include:
- Acylcarnitines
- Amino acids
- Organic acids
- Malonyl CoA and Acetyl CoA
- Pyridine and Adenine Nucleotides: Oxidized (13) OR Reduced Panels (NADH and NADPH)
- Pyridine and Adenine Nucleotides: Oxidized AND Reduced Panels (15)
- Tryptophan Metabolites
- 1-Carbon metabolites
These are performed for a variety of matrices including cellular extracts, plasma or tissue using mass spectrometry. New assays will be developed based on:
- Demand and/or interest in other metabolite subsets and
- Follow-up method development to validate putative metabolites identified by prior global metabolomics pursuits.
The targeted metabolomics employs MS with selected reaction monitoring (SRM) using either a direct infusion approach or after chromatographic separation (UHPLC or GC).
Biomarker identification is performed with accurate mass and fine mass resolution, tandem MS (MS/MS) provided by Core 3 and existing metabolomic databases (Metlin, ChemSpider, pubChem). Through constant collaboration, SECIM continues to grow the library of known metabolites.
Special emphasis in Core 1 is placed on developing SOPs for proper collection/storage of specimens and sample processing prior to MS and NMR. However, interaction with Core 3 provides a mechanism to improve the overall global metabolomics platform as new technologies are developed. An example of this is Isotopic Ratio Outlier Analysis (IROA), a collaborating technology offered through SECIM services.
TO REQUEST SERVICES OR schedule a consultation:
Contact Us
Partners
Chris Beecher, IROA Tech